Plant breeding is the science of changing the traits of plants in order to produce desired characteristics. The production of haploids and doubled haploids dhs through gametic embryogenesis allows a singlestep development of complete homozygous lines from heterozygous parents, shortening the time required to produce homozygous plants in comparison with the. Improved production of doubled haploids of winter and spring. Research on this technique is essential to develop and improve the production of dh lines grown under tropical conditions. Maluszynski m, kasha kj, forster bp, szarekjo i eds doubled haploid production in crop plants. The production of albino plantlets is a serious problem. Fortyfour protocols of doubled haploid production dh are presented in this manual. Agronomy free fulltext haploid and doubled haploid. Inducer colchicine treatment haploid seedlings n doubled haploid plants 2n selfing doubled haploid lines donor. Request pdf doubled haploid production in crop plants. Haploid production free download as powerpoint presentation. Formation of albinos especially with cereals and effect the loss of plants due to albinism. Spontaneous haploid plants are reported in several crop species, like tomato, potato, soybean, maize, barley, wheat, rice, rye etc.
However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also. It is currently the method of choice for inbred line development in many commercial maize breeding programs. Haploid plants are characterized by possessing only a single set of chromosomes gametophytic number of chromosomes i. There is no report yet to achieve the haploid plants from dmsw induced by maize inbred line and especially the corn inducer. Anther culture for haploid and doubled haploid production. Everyday low prices and free delivery on eligible orders. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Accelerated generation of selfed pure line plants for gene. Artificial production of doubled haploids is important in plant breeding. Doubled haploids as a material for biotechnological.
Double haploid a plant or line obtained by doubling the chromosome number of a haploid plant or individual the genetic upgradation of crops through conventional breeding approaches require longer time so there is a need to assist these methods following certain biotechnological tools to shorten. Haploid production in twelve wheat f 1 populations significant genotype differences were found in f 1 crosses for the efficiency of haploid production, based on the percentage of embryo germination and the percentage of haploid green plants regenerated roane, a scabtolerant variety, and pioneer 2684 were the. Introduction the use of haploid plants in the breeding was possible after detection of the line stock 6 coe, 1959, which has the ability to induce maternal haploids with a frequency of 2%, while the appearance through the natural way of the maize haploids is 0. Role of double haploids in crop improvement authorstream presentation. A manual from springer the production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. Dh plants are produced by doubling the chromosome number of a haploid plant, while the conventional homo zygous inbred lines are developed by selfing in.
Breeding and research silviu gabriel mure eanu1, georgeta dicu2, doru ioan marin1, valeriu rotarenco2 1university of agronomic sciences and veterinary medicine of bucharest, 59 m r uti blvd. Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. Haploids are defined as plants with a single chromosome set n and dhs as 100% homozygous individuals stemming from chromosome doubled haploids 2 n. The objective of this study was to determine whether variation in anther culture media would affect the yield of haploids. By selfing the fertile plants, seeds of doubled haploid lines are derived. Production of haploids and doubled haploids in maize. Haploid induction rates were determined following testcrosses to wildtype plants. Seed production from doubled haploid lines in the d 0 nursery.
Plant breeding can be accomplished through many different techniques ranging from simply selecting plants with desirable characteristics for propagation, to methods that make use of knowledge of. Doubled haploid production in crop plants springerlink. This maximum could be approached with the introduction of elite f1 varieties. Haploid production will facilitate reverse breeding, a method that downregulates recom. Production of haploids and doubled haploids haploids produced from diploid species 2n2x, known as monoploids, contain only one set of chromosomes in the sporophytic phase 2nx. Msap profiling suggested that alterations in cytosine methylation occurred as a result of both the.
Prepared by the joint faoiaea programme of nuclear techniques in food and agriculture, plant breeding and genetics laboratory, seibersdorf, 2014. Rnai lines were complemented by acgreentailswapcenh3 or acgreencenh3 transgenes. Maternal haploids are preferentially induced by cenh3. Read this article to learn about the production of haploid plants. Haploid induction requires taking pollen from a haploid inducer plant and putting it on any female ear of corn. The effects of the genotypes of donor plants and three steps of culture media induction, differentiation, and regeneration media were evaluated in unpollinated ovary culture of cucumber cucumis sativus l. It can be done cheaply in the field and is broadly applicable across all genetic starting material. Embryolike structure els formation was significantly affected by.
They can originate spontaneously in nature or as a result of various induction techniques. Chan department of plant biology, university of california, davis, 1 shields avenue, davis, ca 95616, usa. Improvements in the production of doubled haploids in durum wheat. Optimization of doubled haploid production in maize zea mays l.
Anther culture has been traditionally employed for producing haploids in numerous crop plants. Dh has been discovered in at least 200 plant species and is widely. Double haploids are plants those carry two sets of chromosomes that are created from the haploid plants. Production of homozygous diploid by doubling the chromosome number of haploid in vitro makes a pure line in single step and such homozygous pure line is of great importance in plant breeding. Doubled haploids in crop improvement crops plant breeding. Production of doubled haploid wheat lines triticum. Scribd is the worlds largest social reading and publishing site. Chromosome doubling of microsporederived plants is an important factor in the practical application of microspore culture technology because breeding programs require a large number of genetically stable, homozygous doubled haploid plants with a high level of fertility. The importance of haploid plants in basic research and practical plant breeding is well documented. This study was undertaken to develop an efficient method for the production of androgenetic haploids in maize. During the last years, our aim has been focused on the establishment of an efficient protocol for the production of doubled haploid plants from a wide range of genotypes, as well as from f1. The present study represents the first report of the production of haploid and doubled haploid chrysanthemum plants derived from the in vitro culture of nonfertilized ovules, morphological traits were changed significantly in the haploid plants. Once established, the method allows production of doubled haploid lines in large numbers at low costs.
We assessed the ability of a gynogenetic haploid inducer system to. The ability to produce haploid plants is a tremendous asset in genetic and plant breeding studies. Pdf in situ parthenogenetic doubled haploid production. The production of doubled haploids dh has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop. From springer doubled haploid production in crop plants. Furthermore, within a species, differences exist in the ability to produce haploid plants. Doubled haploids as a material for biotechnological manipulation and as a modern tool for breeding oilseed rape 9 snowdon, 2009. Doubled haploid production heartland plant innovations. Protocol for producing doubled haploid plants from anther. An improved protocol for carrot haploid and doubled haploid plant production using induced parthenogenesis and ovule excision in vitro agnieszka kielkowska, adela adamus, and rafal baranski institute of plant biology and biotechnology, faculty of horticulture, university of agriculture in krakow, al. Mar 10, 2015 colchicine treatment concentration mgl colchicine treatment duration h number of regenerated plants number of doubled haploid plants 0 control 0 53 0 125 12 49 10 24 37 9 36 18 8 250 12 41 24 42 27 36 15 9 500 12 17 7 24 14 8 36 9 6 12 0 0 24 0 0 36 0 0 18.
A key question in applying doubled haploid technology in maize breeding is the generation during which haploids should be induced. Todays use of haploids in corn plant breeding sciencedirect. Dwarf male sterile wheat dmsw and corn inducer are potential important germplasm for wheat breeding by recurrent selection and doubled haploid strategies. Request pdf doubled haploid production in crop plants after many years of different experiments around the world on haploid production different methods have become available to the breeders. There are two approaches for the production of haploid plants. Doubled haploid production in crop plants a manual m. However, durum wheat does not respond very well to anther culture. Pages 5358 in doubled haploid p roduction in crop plants. Review on concept and impact of double haploid techniques. Doubled haploids dhs are plants derived from a single pollen grain and doubled artificially to form homozygous diploids.
A scientific explanation of the doubled haploid dh technique, including a step by step guide for the creation of a dh line. Crop science abstract improvement of anther culture methods. Colchicine application during in vitro anther cultures. The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. Treated seedlings are then taken care of at the greenhouse and subsequently transplanted at the field nursery. Doubled haploid production in crop plants a manual edited by m. History of haploids and double haploid production the history of doubled haploids dhs began with the observation of natural sporophytic haploid in the higher plant, datura stramonium. Role of double haploids in crop improvement authorstream. Everything you need to know about doubled haploids in crop improvement. Production of haploids and doubled haploids in oil palm bmc.
Coventry j, kott l, beversdorf wd 1988 manual for microspore culture. Chromosomal doubling treatments impose further stress, thereby increasing the mortality of seedlings in the greenhouse andor in the field. It is not economically viable for haploid production. A dh individual has two identical homologs, so that the amount of recombination information is equivalent to a backcross. The practical protocols are supplemented with a list of published protocols for other crop plants, and separate chapters deal. Kato 2002 treated haploid plants at the sixleaf stage with nitrous oxide gas for 2 days at 600 kpa. To test if haploid inducer lines can be engineered into crops, cenh3 and cenh3. The production of maize doubled haploid dh lines is a technique commonly used by private companies, but not by brazilian public institutions. The ploidy level could be doubled autonomously or by chemical. Haploid plants are generally weak and show susceptibility to various biotic and abiotic stresses mahuku 2012. Here we present the first highthroughput screen to identify. The anther culture procedure, however, can only become an efficient, economical part of breeding practice if it ensures the production of a sufficient number of cytologically stable doubled haploid plants from a wide range of genotypes. Since the first report of haploid production in 1920s, the dh, both the range of species and. Crop science abstract generation of haploids in maize.
Dh maize was first identified by stadler and randolph 1932. Thomas lubberstedt, comajor professor candice gardner, comajor professor. Risk of chimera and callus formation from anther wall. Optimum hybrid maize breeding strategies using doubled haploids. In research, doubled haploid lines have been used for structural and functional genomics, markertrait association studies, and molecular cytogenetics chang and coe, 2009. Haploid plant production via androgenesis has been very limited or nonexistent in many plant species. Doubled haploid production in wheat through wide hybridization.
This is the first manual presenting a set of protocols for production of doubled haploids dh in 22 major crop plant species including 4 tree species. The production of haploids and doubled haploids dhs through gametic embryogenesis allows a singlestep development of complete homozygous lines from heterozygous parents, shortening the time required to produce homozygous plants. Production and subsequent conversion of haploids into doubled haploids has many applications in genetics and plant breeding dunwell 2010. Among the most promising emerging technologies is the production of doubled haploids genetically pure plant lines that offer a quick. It has been used to improve the quality of nutrition in products for humans and animals. In practice, f 1derived doubled haploids denoted by dh are typically produced in maize rober et al.
A manual fortyfour protocols of doubled haploid production dh are presented in this manual. Frontiers doubled haploid production from spanish onion. This technique is simple and used in the production of b. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding. Jan 25, 2015 the development and production of haploid plant in vitro is very important for the study of fundamental and applied aspects of genetics in the higher plants. The development of such elite lines has thus far been prevented by difficulties in generating homozygous parental types for f1 generation. Even within an amenable species, such as tobacco, some genotypes produce haploids at a much higher rate than do others.
The yield of haploid production in wheat triticum aestivum l. The preferred method for the production of haploid or dh plants differs per species and depends on protocol availability, as well as efficiency in terms of investments and yields. In addition, doubling the chromosome number of a haploid to produce a doubled haploid results in a completely homozygous plant. Doubled haploid technology for line development in maize. Chromosome doubling of microsporederived plants from. Double haploid technology refers to the use of the microspore or anther culture ovary culture in few species to obtain haploid embryos from microspores.
Pdf doubled haploids in cucurbit species are produced through in situ parthenogenesis via pollination with irradiated pollen for further use as. Haploids are plants that contain a gametic chromosome number n. If you wish more information about this publication, then please email to p. Oil palm is the worlds most productive oilfood crop despite yielding well below its theoretical maximum. Doubled haploidy and induced mutation springerlink.
The in vivo haploid induction approach offers several advantages compared to the in vitro induction approach and recurrent selfpollination. Optimization of doubled haploid production in maize zea. They are smaller and exhibit a lower plant vigour compared to donor plants and are sterile due to the inability of their chromosomes to pair during meiosis. Haploid is the general term for plants sporophytes that contain the gametic chromosome number n. Production of doubled haploids will be the quickest if haploids are induced among f 1 plants. It contains various protocols and approaches of dh production proven for different germplasm of the same species. Haploid seeds collected in different induction crosses until 2012, were used for colchicine doubling treatment. Using the cross of wheat and maize is a very useful way to produce wheat haploid plants by chromosome elimination. Stages of plant life cycle at that haploid plant can produce 1. Tissue or callus comprises a chimera of diploid, tetraploid and haploid cells.
Doublehaploid induction speeds up plantbreeding process. There are at least three critical steps involved in dh line production. A doubled haploid dh is a genotype formed when haploid cells undergo chromosome doubling. Doubled haploid production for thousands of years, farmers have worked to improve their crop varieties, slowly breeding new traits over many generations.
Characterization of in vitro haploid and doubled haploid. The doubled haploid dh derived from hybrid progenies can be used as recombinant line with favorable gene combinations bentolila et al. In view of the numerous advantages of doubled haploids in maize breeding, in vivo haploid. The most efficient way to produce doubled haploids in corn is through haploid induction, he adds. Doubled haploid production in crop plants request pdf. Here, we describe the in vivo approach for generation of maternal doubled haploids dhs.
The protocols describe in detail all steps of dh production from donor plants growth conditions, through in vitro procedures. An improved protocol for carrot haploid and doubled. Development of maize haploid inducer lines and doubled. Production and identification of doubled haploids in tropical.
From the time of accidental but i mmensely valuable discovery of androgenic haploidy in 1964 by guha and maheshwari 10 and production of rice haploids in 1968 by niizeki and oono 11, impressive a dvances have been made in several laboratories throughout the world. Doubled haploid production in crop plants a manual, plant. Genome mapping, a recent development in molecular biology, can be more conveniently achieved by using doubled haploid plant species. They are related to at least 33 plant species, not counting protocols on production of dh from interspecific. Doubled haploids refer to the diploid lines which are obtained by doubling the chromosome number of a haploid line by colchicine treatment. Today, advanced plant breeding technologies enable far more significant improvements in far less time. Role of doubled haploids in vegetable crop improvement. All steps of doubled haploid production are detailed from donor plant growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. Should maize doubled haploids be induced among f 1 or f2 plants. Production and identification of haploid dwarf male sterile. A comparison of di haploids doubled haploids with diploid wild plant species will be useful to trace the evolutionary origin of various plants. Haploids are plants with a gametophytic chromosome number and doubled haploids are haploids that have undergone chromosome duplication.
Artificial production of doubled haploids is important in plant breeding haploid cells are produced from pollen or egg cells or from other cells of the gametophyte, then by induced or spontaneous chromosome doubling, a doubled haploid cell is produced, which can be grown into a doubled haploid plant. Novel technologies in doubled haploid line development ncbi. The use of doubled haploids in onion breeding is limited due to the low gynogenesis efficiency of this species. Pdf haploids and doubled haploids in plant breeding.1129 293 869 590 549 617 1007 549 254 818 328 1404 134 1016 871 1465 903 647 568 1497 1140 271 354 155 1123 1041 274 1479 594 631 206 1452 157 578 1200 461 659 1435 1162 636 1398 312 1266 1077 1008 181